pSV Sport SREBP-1c
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8883||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3160
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1200
MutationActive version of SREBP-1c.
Entrez GeneSrebf1 (a.k.a. ADD1, SREBP1, bHLHd1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Note that this is a mouse SREBP-1c, while the paper reference is a rat SREBP-1c. SREBP-1c differs from SREBP1a over amino acids 3-5 and contains a deletion of amino acids 6-29. See Figure 1c of the associated publication for more information.
Addgene's sequencing results identified a deletion of aa90-131 of SREBP1c compared to GenBank sequence NP_035610.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSV Sport SREBP-1c was a gift from Bruce Spiegelman (Addgene plasmid # 8883 ; http://n2t.net/addgene:8883 ; RRID:Addgene_8883)
For your References section:ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation. Tontonoz P, Kim JB, Graves RA, Spiegelman BM. Mol Cell Biol. 1993 Aug . 13(8):4753-9. PubMed 8336713