168,579 results

1. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-HA-UCHL1

   Plasmid

   #22563

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   UCHL1 (UCHL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAY5 (mAID-EGFP-NLS piggyBac)

   Plasmid

   #140532

   Purpose

   mAID-EGFP-NLS piggyBac

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHaloTag-EGFP

   Plasmid

   #86629

   Purpose

   Expresses HaloTag-eGFP tandem (cytosolic)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   HaloTag
   

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #69138

   Purpose

   For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

   Depositor

   Takuji Iwasato

   Article

   Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

   Insert

   CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
   

   Tags

   none

   Expression

   Mammalian

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR_attB(bxb)_lox

   Plasmid

   #183762

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Man1B1-GFP

   Plasmid

   #163646

   Purpose

   Expresses GFP tagged Man1B1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mannosidase alpha class 1B member 1 (MAN1B1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmU6-gRNA

   Plasmid

   #53187

   Purpose

   Expresses the S. pyogenes sgRNA from the mouse U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-mitoLbNOX

    Plasmid

    #74448

    Purpose

    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    mitoLbNOX
    

    Tags

    Flag tag with linker and mitochondrial targeting …

    Mutation

    Human codon optimized

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO-EGFP (AAV8)

    Viral Prep

    #50457-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SepRS(2)/pSertRNA(B4)/EF-Sep

    Plasmid

    #173897

    Purpose

    The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

    Depositor

    Jason W Chin

    Article

    Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

    Inserts

    SepRS(2)
    pSertRNA
    EF-Sep
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER21 (ORF-EG)

    Plasmid

    #46948

    Purpose

    Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

    Depositor

    Stephen Elledge, Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)
    

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPOTv6-puro-3Ty:mNG:3Ty

    Plasmid

    #179798

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean, Keith Gull

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mNG
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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