PurposeTo overexpress PTEN in Mammalian Cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78776||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA PTEN was a gift from Jaewhan Song (Addgene plasmid # 78776 ; http://n2t.net/addgene:78776 ; RRID:Addgene_78776)
For your References section:PI3K/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis. Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, Chung JY, Lee C, Cho H, Song J. Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769. 10.1038/ncomms8769 PubMed 26183061