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Tunable protein degradation in bacteria.
Cameron DE, Collins JJ
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
75439pZA16mflonPlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
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75441pECGMC3Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3
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75445pECM1Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75446pECT1Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75447pECT1BPlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75448pECT1DPlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75449pECT1EPlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75450pECT3Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75451pECT5Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
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75452pZE27GFPPlasmid for constitutive expression of gfp
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75453pZE27GFP0Plasmid for constitutive expression of gfp-pdt
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75454pZE27GFP1Plasmid for constitutive expression of gfp-pdt1
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75455pZE27GFP2Plasmid for constitutive expression of gfp-pdt2
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75456pZE27GFP3Plasmid for constitutive expression of gfp-pdt3
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75457pZE27GFP4Plasmid for constitutive expression of gfp-pdt4
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75458pZE27GFP5Plasmid for constitutive expression of gfp-pdt5
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75459pZE27GFP3APlasmid for constitutive expression of gfp-pdt3A
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75460pZE27GFP3BPlasmid for constitutive expression of gfp-pdt3B
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75461pZE27GFP3CPlasmid for constitutive expression of gfp-pdt3C
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75462pZE27GFP3DPlasmid for constitutive expression of gfp-pdt3D
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75463pZE27GFP3EPlasmid for constitutive expression of gfp-pdt3E
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75464pZE27MC3Plasmid for constitutive expression of mcherry-pdt3
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75465pECJ3Toggle switch plasmid containing lacI-pdt3
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75466pECJ3BToggle switch plasmid containing lacI-pdt3B
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75467pECJ3DToggle switch plasmid containing lacI-pdt3D
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Antibodies from Article