PurposePlasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75441||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
- Promoter nisA promoter
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site AatII (not destroyed)
- 5′ sequencing primer AGTCTTATAACTATACTGACAATAG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pECGMC3 was a gift from James Collins (Addgene plasmid # 75441 ; http://n2t.net/addgene:75441 ; RRID:Addgene_75441)
For your References section:Tunable protein degradation in bacteria. Cameron DE, Collins JJ. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17. 10.1038/nbt.3053 PubMed 25402616