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(Plasmid #84653)


Item Catalog # Description Quantity Price (USD)
Plasmid 84653 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Professor Sheng Yang (Plasmid 62225)
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 14300
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9 nickase
  • Species
    S. pyogenes
  • Insert Size (bp)
  • Mutation
  • GenBank ID

Gene/Insert 2

  • Gene/Insert name
  • Insert Size (bp)

Gene/Insert 3

  • Gene/Insert name
    homology arms of LC2W_2179
  • Insert Size (bp)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCNICK was a gift from Sheng Yang (Addgene plasmid # 84653 ; ; RRID:Addgene_84653)
  • For your References section:

    CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Song X, Huang H, Xiong Z, Ai L, Yang S. Appl Environ Microbiol. 2017 Sep 1. pii: AEM.01259-17. doi: 10.1128/AEM.01259-17. 10.1128/AEM.01259-17 PubMed 28864652