PurposeMiddle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||82588||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 4206
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
- Promoter None
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid was also used in Fowler et al., 2016 (PMID: 27500400) and is part of the associated kit.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME-GAL4-ERT2-VP16 was a gift from Kryn Stankunas (Addgene plasmid # 82588 ; http://n2t.net/addgene:82588 ; RRID:Addgene_82588)
For your References section:Spatial and temporal control of transgene expression in zebrafish. Akerberg AA, Stewart S, Stankunas K. PLoS One. 2014 Mar 18;9(3):e92217. doi: 10.1371/journal.pone.0092217. eCollection 2014. PONE-D-13-46380 [pii] PubMed 24643048