|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24334||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeWorm Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
MutationmCherry driven by an HSP70 promoterCerulean driven by a CrystalineA promoter*
/ Fusion Protein
- GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SV40pA (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean was a gift from Didier Stainier (Addgene plasmid # 24334 ; http://n2t.net/addgene:24334 ; RRID:Addgene_24334)
For your References section:Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Hesselson D, Anderson RM, Beinat M, Stainier DY. Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901. 10.1073/pnas.0906348106 PubMed 19706417