Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA.
Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch PJ, Kagan JC
Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 10.1016/j.cell.2019.01.049. Epub 2019 Feb 28. PubMed Article
Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch PJ, Kagan JC
Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 10.1016/j.cell.2019.01.049. Epub 2019 Feb 28. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130908 | pRRL-gRNA(anti-cGAS)-Cas9-Puro | gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker |
| 130910 | pLenti-CMV-cGAS-HA | Expresses human cGAS-HA; Puromycin selection marker |
| 130912 | pLenti-CMV-cGASΔN-HA | Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker |
| 130913 | pLenti-CMV-cGAS-N-HA | Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker |
| 130915 | pLenti-CMV-cGASΔN-HA (murine) | Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker |
| 130916 | pLenti-CMV-cGAS-N-HA (murine) | Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker |
| 130917 | pLenti-CMV-cGAS C396/7A-HA | Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker |
| 130918 | pLenti-CMV-cGASΔN C396/7A-HA | Expresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection marker |
| 130919 | pLenti-CMV-cGAS R71/75E-HA | Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker |
| 130920 | pLenti-CMV-cGAS R71/75E C396/7A-HA | Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker |
| 130921 | pLenti-CMV-TIRAP-cGASΔN-HA | Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker |
| 130922 | pLenti-CMV-Fyn-cGASΔN-HA | Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker |