|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79144||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepSpCas9(BB)-2A-GFP (PX458)
Backbone manufacturerAddgene #48138
- Total vector size (bp) 10243
Modifications to backboneReplaced CBh promoter with CAG promoter
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
- Promoter CAG
/ Fusion Protein
- 2A-GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (not destroyed)
- 3′ cloning site BbsI (not destroyed)
- 5′ sequencing primer ACTATCATATGCTTACCGTAAC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThis plasmid was modified from Addgene plasmid #48138 and contains GFP cassette.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79144 ; http://n2t.net/addgene:79144 ; RRID:Addgene_79144)