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Purposeexpression of mGM-CSF in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5060
- Total vector size (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegranulocyte-monocyte colony-stimulating factor
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Alt nameCsf2
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SpeciesM. musculus (mouse)
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Insert Size (bp)442
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GenBank IDNM_009969
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Entrez GeneCsf2 (a.k.a. CSF, Csfgm, GMCSF, Gm-CSf, MGI-IGM)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was derived by PCR and T/A cloning.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR3.1-mGM-CSF was a gift from Loree Heller (Addgene plasmid # 74465 ; http://n2t.net/addgene:74465 ; RRID:Addgene_74465) -
For your References section:
In vivo electroporation of plasmids encoding GM-CSF or interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long-term antitumour immunity. Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R. Melanoma Res. 2000 Dec;10(6):577-83. PubMed 11198480