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pCR3.1-mGM-CSF
(Plasmid #74465)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74465 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCR3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5060
  • Total vector size (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    granulocyte-monocyte colony-stimulating factor
  • Alt name
    Csf2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    442
  • GenBank ID
    NM_009969
  • Entrez Gene
    Csf2 (a.k.a. CSF, Csfgm, GMCSF, Gm-CSf, MGI-IGM)
  • Promoter CMV

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

This plasmid was derived by PCR and T/A cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3.1-mGM-CSF was a gift from Loree Heller (Addgene plasmid # 74465 ; http://n2t.net/addgene:74465 ; RRID:Addgene_74465)
  • For your References section:

    In vivo electroporation of plasmids encoding GM-CSF or interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long-term antitumour immunity. Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R. Melanoma Res. 2000 Dec;10(6):577-83. PubMed 11198480