|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74467||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5060
- Total vector size (bp) 5635
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameinterleukin 2
SpeciesM. musculus (mouse)
Insert Size (bp)575
Entrez GeneIl2 (a.k.a. Il-2)
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCR3.1-mIL2 was a gift from Loree Heller (Addgene plasmid # 74467 ; http://n2t.net/addgene:74467 ; RRID:Addgene_74467)
For your References section:In vivo electroporation of plasmids encoding GM-CSF or interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long-term antitumour immunity. Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R. Melanoma Res. 2000 Dec;10(6):577-83. PubMed 11198480