Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #124372)


Item Catalog # Description Quantity Price (USD)
Plasmid 124372 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pLenti X1 Puro DEST (Addgene #17297)
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman
  • Backbone size w/o insert (bp) 9031
  • Total vector size (bp) 8646
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Anguilla japonica
  • Insert Size (bp)
  • Promoter HRE (HIF responsive element 5x + CMV minimal promoter)
  • Tag / Fusion Protein
    • PEST-degron and Myc tag (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Primer 1 CACCGTACCGAGCTTTTCTCGAGC
  • 3′ sequencing primer Primer 2 CAGCTGGTTCTTTCCGCCTC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    the HRE-UnaG insert was obtained from a plasmid that we received with an MTA from the Max-Planck Institute Münster, Germany

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-HRE-dUnaG was a gift from Roland Friedel (Addgene plasmid # 124372 ; ; RRID:Addgene_124372)
  • For your References section:

    Hypoxic niches attract and sequester tumor-associated macrophages and cytotoxic T cells and reprogram them for immunosuppression. Sattiraju A, Kang S, Giotti B, Chen Z, Marallano VJ, Brusco C, Ramakrishnan A, Shen L, Tsankov AM, Hambardzumyan D, Friedel RH, Zou H. Immunity. 2023 Jul 7:S1074-7613(23)00274-1. doi: 10.1016/j.immuni.2023.06.017. 10.1016/j.immuni.2023.06.017 PubMed 37451265