HypoxCR
(Plasmid #59946)

• Purpose: A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.
• Depositing Lab: Anne Le
• Publication: Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. pii: 201402012.

Backbone

• Vector backbone: pLVXPuro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8102
• Total vector size (bp): 11603
• Modifications to backbone: Inserted cassette with HRE-GFP-PEST and CMV-mChery-Gem
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: GFP-Pest
• Alt name: GFP
• Species: Synthetic
• Promoter: min CMV
• Tag / Fusion Protein:
  • PEST (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: BGH-rev
• 3′ sequencing primer: Cer-F

Gene/Insert 2

• Gene/Insert name: mCherry-Geminin
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: mCherry-F
• 3′ sequencing primer: PGK-A3

Resource Information

• Supplemental Documents:
  • Protocol from Le Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Le lab has also used this plasmid in the following publication: http://www.pnas.org/content/113/36/E5328.full