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HypoxCR
(Plasmid #59946)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59946 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVXPuro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8102
  • Total vector size (bp) 11603
  • Modifications to backbone
    Inserted cassette with HRE-GFP-PEST and CMV-mChery-Gem
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GFP-Pest
  • Alt name
    GFP
  • Species
    Synthetic
  • Promoter min CMV
  • Tag / Fusion Protein
    • PEST (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mCherry-Geminin
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

The Le lab has also used this plasmid in the following publication: http://www.pnas.org/content/113/36/E5328.full

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HypoxCR was a gift from Anne Le (Addgene plasmid # 59946 ; http://n2t.net/addgene:59946 ; RRID:Addgene_59946)
  • For your References section:

    Tumorigenicity of hypoxic respiring cancer cells revealed by a hypoxia-cell cycle dual reporter. Le A, Stine ZE, Nguyen C, Afzal J, Sun P, Hamaker M, Siegel NM, Gouw AM, Kang BH, Yu SH, Cochran RL, Sailor KA, Song H, Dang CV. Proc Natl Acad Sci U S A. 2014 Aug 11. pii: 201402012. 10.1073/pnas.1402012111 PubMed 25114222