Phage-assisted evolution and protein engineering yield compact, efficient prime editors.
Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR
Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. PubMed Article
Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR
Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207851 | pCMV-PE6a | Mammalian expression of PE6a prime editor |
| 207852 | pCMV-PE6b | Mammalian expression of PE6b prime editor |
| 207853 | pCMV-PE6c | Mammalian expression of PE6c prime editor |
| 207854 | pCMV-PE6d | Mammalian expression of PE6d prime editor |
| 207855 | pCMV-PE6e | Mammalian expression of PE6e prime editor |
| 207856 | pCMV-PE6f | Mammalian expression of PE6f prime editor |
| 207857 | pCMV-PE6g | Mammalian expression of PE6g prime editor |
| 207858 | pCMV-PEmaxRNaseHtrunc | Mammalian expression of PEmaxDRNaseH prime editor |
| 207859 | v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB | AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs |
| 207860 | v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB | AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs |
| 207861 | v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB | AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs |
| 207862 | v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP | AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA |
| 207863 | v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP | AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA |