v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
(Plasmid
#207863)
-
PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonep601 modified
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
-
SpeciesCas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
-
Insert Size (bp)2856
-
MutationSee Manuscript
- Promoter Cbh
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGCGAGTCAGTGAGCGAG
- 3′ sequencing primer GTTTTCCCAGTCACGAC (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP was a gift from David Liu (Addgene plasmid # 207863 ; http://n2t.net/addgene:207863 ; RRID:Addgene_207863) -
For your References section:
Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR. Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. 10.1016/j.cell.2023.07.039 PubMed 37657419
