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Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, Marshall JL, Chen F
Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138586pUC19 - T7 pro - IRES - EGFPExpress EGFP with an upstream T7 promoter in pUC19 backbone
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138610pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamatoExpress TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone
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138615pLX - T7pro - EBFPExpress EBFP with an upstream T7 promoter in a lentiviral backbone
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Recombinant Antibodies from Article