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pUC19 - T7 pro - IRES - EGFP
(Plasmid #138586)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 138586 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    720

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    EGFP was cloned from pcDNA3.1(+)IRES GFP, which was obtained from Kathleen_L Collins (Addgene plasmid # 51406.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the depositor sequence linked in the supplemental documents section is different compared to the Addgene verified sequence. We recommend viewing the Addgene verified sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19 - T7 pro - IRES - EGFP was a gift from Fei Chen (Addgene plasmid # 138586 ; http://n2t.net/addgene:138586 ; RRID:Addgene_138586)
  • For your References section:

    Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, Marshall JL, Chen F. Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8. 10.1038/s41587-019-0331-8 PubMed 31844291