pUC19 - T7 pro - IRES - EGFP
PurposeExpress EGFP with an upstream T7 promoter in pUC19 backbone
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138586||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3844
- Total vector size (bp) 4564
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)720
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the depositor sequence linked in the supplemental documents section is different compared to the Addgene verified sequence. We recommend viewing the Addgene verified sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC19 - T7 pro - IRES - EGFP was a gift from Fei Chen (Addgene plasmid # 138586 ; http://n2t.net/addgene:138586 ; RRID:Addgene_138586)
For your References section:Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, Marshall JL, Chen F. Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8. 10.1038/s41587-019-0331-8 PubMed 31844291