pUC19 - T7 pro - IRES - EGFP
(Plasmid #138586)

• Purpose: Express EGFP with an upstream T7 promoter in pUC19 backbone
• Depositing Lab: Fei Chen
• Publication: Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 3844
• Total vector size (bp): 4564

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Insert Size (bp): 720

Resource Information

• Supplemental Documents:
  • pUC19-T7 pro-IRES-EGFP.gb
• A portion of this plasmid was derived from a plasmid made by: EGFP was cloned from pcDNA3.1(+)IRES GFP, which was obtained from Kathleen_L Collins (Addgene plasmid # 51406.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the depositor sequence linked in the supplemental documents section is different compared to the Addgene verified sequence. We recommend viewing the Addgene verified sequence.