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(Plasmid #16716)


Item Catalog # Description Quantity Price (USD)
Plasmid 16716 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Protein Kinase A
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    Dominant negative. Mutations at cAMP binding sites.
  • Entrez Gene
    Prkar1a (a.k.a. 1300018C22Rik, RIalpha, Tse-1, Tse1)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.

Plasmid History:
Constructed by Anne Ungar in 1996.

Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M7 pdnPKA-GFP was a gift from Randall Moon (Addgene plasmid # 16716 ; ; RRID:Addgene_16716)
  • For your References section:

    Inhibition of protein kinase A phenocopies ectopic expression of hedgehog in the CNS of wild-type and cyclops mutant embryos. Ungar AR, Moon RT. Dev Biol. 1996 Aug 25;178(1):186-91. doi: 10.1006/dbio.1996.0209. 10.1006/dbio.1996.0209 PubMed 8812120