|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16716||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeXenopus Expression
Growth in Bacteria
Alt nameProtein Kinase A
SpeciesM. musculus (mouse)
MutationDominant negative. Mutations at cAMP binding sites.
Entrez GenePrkar1a (a.k.a. 1300018C22Rik, RIalpha, Tse-1, Tse1)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.
Constructed by Anne Ungar in 1996.
Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M7 pdnPKA-GFP was a gift from Randall Moon (Addgene plasmid # 16716 ; http://n2t.net/addgene:16716 ; RRID:Addgene_16716)