|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15222||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCREB S133A
SpeciesR. norvegicus (rat)
Insert Size (bp)1000
MutationSerine 133 mutated to Alanine
Entrez GeneCreb1 (a.k.a. Creb)
/ Fusion Protein
- Gal4 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/SalI (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Gal4-N-term primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Wild-type CREB S133A fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the CREB S133A as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGAL4-CREB S133A was a gift from Ugo Moens (Addgene plasmid # 15222 ; http://n2t.net/addgene:15222 ; RRID:Addgene_15222)
For your References section:The cAMP signalling pathway activates CREB through PKA, p38 and MSK1 in NIH 3T3 cells. Delghandi MP, Johannessen M, Moens U. Cell Signal. 2005 Nov . 17(11):1343-51. 10.1016/j.cellsig.2005.02.003 PubMed 16125054