pCalpha EV (PKA catalytic subunit Calpha)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15310||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneZem3 (pUC13 + MT prom + hGH polyA)
Backbone manufacturerDr. Richard Palmiter
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namecAMP-dependent protein kinase catalytic subunit alpha
SpeciesM. musculus (mouse)
Insert Size (bp)1318
Entrez GenePrkaca (a.k.a. PKCD, Pkaca)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCalpha EV was constructed by isolating the 1318 base pair NaeI/PvuII fragment of pMCalpha and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA insert.
The coding sequence can be cut out with NcoI and ApaI, which should release a 1152bp fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCalpha EV (PKA catalytic subunit Calpha) was a gift from Stanley McKnight (Addgene plasmid # 15310 ; http://n2t.net/addgene:15310 ; RRID:Addgene_15310)
For your References section:Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase. Uhler MD, McKnight GS. J Biol Chem. 1987 Nov 5. 262(31):15202-7. PubMed 3667630
Map uploaded by the depositor.