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pCalpha EV (PKA catalytic subunit Calpha)
(Plasmid #15310)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 15310 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Zem3 (pUC13 + MT prom + hGH polyA)
  • Backbone manufacturer
    Dr. Richard Palmiter
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    cAMP-dependent protein kinase catalytic subunit alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Prkaca (a.k.a. PKCD, Pkaca)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCalpha EV was constructed by isolating the 1318 base pair NaeI/PvuII fragment of pMCalpha and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA insert.

The coding sequence can be cut out with NcoI and ApaI, which should release a 1152bp fragment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCalpha EV (PKA catalytic subunit Calpha) was a gift from Stanley McKnight (Addgene plasmid # 15310 ; ; RRID:Addgene_15310)
  • For your References section:

    Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase. Uhler MD, McKnight GS. J Biol Chem. 1987 Nov 5. 262(31):15202-7. PubMed 3667630