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pCbeta EV (PKA catalytic subunit Cbeta)
(Plasmid #15311)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15311 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Zem3 (pUC13 + MT prom + hGH polyA)
  • Backbone manufacturer
    Dr. Richard Palmiter
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKACB
  • Alt name
    cAMP-dependent protein kinase catalytic subunit beta
  • Alt name
    PKACB
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1246
  • Entrez Gene
    Prkacb (a.k.a. CbPKA, Pkacb)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCbeta EV was constructed by isolating the 1246bp SacI fragment of pMCbeta and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA fragment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCbeta EV (PKA catalytic subunit Cbeta) was a gift from Stanley McKnight (Addgene plasmid # 15311 ; http://n2t.net/addgene:15311 ; RRID:Addgene_15311)
  • For your References section:

    Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase. Uhler MD, McKnight GS. J Biol Chem. 1987 Nov 5. 262(31):15202-7. PubMed 3667630