Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins.
Jin F, Shen C, Wang Y, Wang M, Sun M, Hattori M
Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y. PubMed Article
Jin F, Shen C, Wang Y, Wang M, Sun M, Hattori M
Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159986 | mEGFP-NbALFA | For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA) |
| 159987 | mCherry-NbALFA | For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA) |
| 159988 | pETNb-nALFA | For the protein expression with ALFA tag at N-terminus in E. coli |
| 159989 | pETNb-cALFA | For the protein expression with ALFA tag at C-terminus in E. coli |
| 159990 | pFBNb-cALFA | For the protein expression with ALFA tag at C-terminus in insect cells |
| 159991 | pBMNb-cALFA | For the protein expression with ALFA tag at C-terminus in mammalian cells |
| 160498 | BbZIP-cALFA | For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli |
| 160499 | hP2X3-cALFA | For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells |