BbZIP-cALFA
(Plasmid #160498)

• Purpose: For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli
• Depositing Lab: Motoyuki Hattori
• Publication: Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y.

Backbone

• Vector backbone: pETNb-cALFA
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BbZIP
• Species: Synthetic
• Tag / Fusion Protein:
  • HRV3C protease cleavage site-ALFA tag-8histidine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry