PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122151||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
- Total vector size (bp) 15861
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namedCas9 fused to SunTag(24x)
Insert Size (bp)6909
- Promoter SFFV
/ Fusion Proteins
- NLS (N terminal on insert)
- 2xNLS-P2A-BFP (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
This plasmid is the same as Addgene_60910 except for the dSV40 promoter was replaced with SFFV promoter. Each of the 24 copies of GCN4_v4 peptide can be recognized by scFv-GCN4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE was a gift from Clifford Brangwynne (Addgene plasmid # 122151 ; http://n2t.net/addgene:122151 ; RRID:Addgene_122151)
For your References section:Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. 10.1016/j.cell.2018.10.057 PubMed 30500535