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Addgene

pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
(Plasmid #122151)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122151 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHR
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 15861
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9 fused to SunTag(24x)
  • Insert Size (bp)
    6909
  • Promoter SFFV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • 2xNLS-P2A-BFP (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Plasmid #60910. (Tanenbaum et al., Cell, 2014)
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is the same as Addgene_60910 except for the dSV40 promoter was replaced with SFFV promoter. Each of the 24 copies of GCN4_v4 peptide can be recognized by scFv-GCN4.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE was a gift from Clifford Brangwynne (Addgene plasmid # 122151 ; http://n2t.net/addgene:122151 ; RRID:Addgene_122151)
  • For your References section:

    Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. 10.1016/j.cell.2018.10.057 PubMed 30500535