pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
(Plasmid #121966)

• Purpose: Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
• Depositing Lab: Clifford Brangwynne
• Publication: Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 10563
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: scFv-GCN4
• Insert Size (bp): 2300
• Promoter: SFFV
• Tag / Fusion Protein:
  • superfold GFP-GB1-NLS-iLID (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: scFv-GCN4 from Addgene Plasmid #60906 (Tanenbaum et al., Cell, 2014) and iLID from Addgene Plasmid #60413 (Guntas et al., PNAS, 2015)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector contains iLID, an engineered LOV2-SsrA protein for light dependent heterodimerization.