PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121966||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
- Total vector size (bp) 10563
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)2300
- Promoter SFFV
/ Fusion Protein
- superfold GFP-GB1-NLS-iLID (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byscFv-GCN4 from Addgene Plasmid #60906 (Tanenbaum et al., Cell, 2014) and iLID from Addgene Plasmid #60413 (Guntas et al., PNAS, 2015)
Terms and Licenses
Vector contains iLID, an engineered LOV2-SsrA protein for light dependent heterodimerization.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE was a gift from Clifford Brangwynne (Addgene plasmid # 121966 ; http://n2t.net/addgene:121966 ; RRID:Addgene_121966)
For your References section:Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. 10.1016/j.cell.2018.10.057 PubMed 30500535