168,567 results

1. pcDNA3.1-GGamma1-GFP2

   Plasmid

   #140989

   Purpose

   Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma1-GFP2 (GNGT1 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

   Pooled Library

   #171531

   Purpose

   Pooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB)

   Depositor

   Chunliang Li

   Article

   Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

3. spNW50

   Plasmid

   #173486

   Purpose

   Expresses spNW50 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW50
   

   Tags

   Histag

   Expression

   Bacterial

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-2xFLAG-SREBP-1c

   Plasmid

   #26802

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   SREBP-1c (SREBF1 Human)
   

   Tags

   2XFLAG

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 deltaN89 Beta-catenin

   Plasmid

   #19288

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-89

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pYC01

   Plasmid

   #224586

   Purpose

   IPTG-inducible phage φX174 lysis gene E

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

   Insert

   phage φX174 lysis gene E (phiX174p08 )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PA1lacO-1

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Stephen Elledge, Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-CD36-C-10

   Plasmid

   #55011

   Purpose

   Localization: Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD36 (CD36 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn-fDIO-taCasp3-TEVp

    Plasmid

    #207997

    Purpose

    Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

    Depositor

    Kazunari Miyamichi

    Article

    Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

    Has Service

    AAV5

    Insert

    taCasp3-TEVp
    

    Use

    AAV

    Promoter

    hSyn promoter

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid

    #140981

    Purpose

    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasL-RLuc8 (GNAS Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-mitoLbNOX

    Plasmid

    #74448

    Purpose

    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    mitoLbNOX
    

    Tags

    Flag tag with linker and mitochondrial targeting …

    Mutation

    Human codon optimized

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIN4-EF1a-ETV2-IRES-Puro

    Plasmid

    #61061

    Purpose

    lentiviral vector for ETV2

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1alpha

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid

    #100843

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LC-E75

    Bacterial Strain

    #115925

    Purpose

    Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

17. MAC_N_STA5A

    Plasmid

    #167799

    Purpose

    Expresses STA5A in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    STA5A (STAT5A Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRPML1-HA

    Plasmid

    #18825

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-DIO-EGFP (AAV8)

    Viral Prep

    #50457-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pSEVA451-Cpf1

    Plasmid

    #192362

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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