164,411 results

1. pcDNA3 myc CIITA (P#808)

   Plasmid

   #14650

   Depositor

   Matija Peterlin

   Article

   Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

   Insert

   CIITA (CIITA Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav-GA

   Plasmid

   #209880

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Use

   Tags

   CAVIN1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG1169 FIREmate-ECFP-Cb5

   Plasmid

   #205167

   Purpose

   Expresses FIREmate-ECFP-Cb5 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Cb5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sumire-pRsetB

   Plasmid

   #193361

   Purpose

   GFP variant emit 414 nm fluorescence.

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   Sumire
   

   Use

   E.coli expression

   Tags

   His X6 tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagRFP-T-Golgi-7

   Plasmid

   #58018

   Purpose

   Localization: Golgi Complex, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1(+)-FLAG-RGS14

   Plasmid

   #236567

   Purpose

   Expresses Flag-RGS14 in mammalian cells

   Depositor

   John Hepler

   Article

   Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

   Insert

   Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2200

   Plasmid

   #143352

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU4F1 (POU4F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-M13-TEV-C-P2A-TdTomato

   Plasmid

   #92391

   Purpose

   produces AAV expressing Cal-light M13-TevC component and TdTomato

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   M13-TEV-C-P2A-TdTomato
   

   Use

   AAV and Synthetic Biology

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-cmyc

   Plasmid

   #16011

   Purpose

   mammalian expression of human cmyc

   Depositor

   Wafik El-Deiry

   Article

   Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

   Insert

   cmyc (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid

    #71293

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV4 p50

    Plasmid

    #21965

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NF-kB p50 subunit (NFKB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-DsRed

    Plasmid

    #11151

    Purpose

    Mammalian expression vector for expression of DsRed

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    DsRed2 from Discosoma sp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-h53BP1 (siRNA resistant)

    Plasmid

    #110301

    Purpose

    Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    GAACGAGGA to GAGCGGGGC

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcGFP1-N1-SialT

    Plasmid

    #87324

    Purpose

    To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AdEasier-1 cells (strain)

    Bacterial Strain

    #16399

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Ampicillin and streptomycin

    Species

    Escherichia coli

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

17. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-double floxed-eNpHR-EYFP-WPRE-pA (AAV9)

    Viral Prep

    #20949-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-double floxed-eNpHR-EYFP-WPRE-pA (#20949). In addition to the viral particles, you will also receive purified pAAV-double floxed-eNpHR-EYFP-WPRE-pA plasmid DNA. EF1a-driven, cre-dependent eNpHR EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCEP4-YU2gp160

    Plasmid

    #100927

    Purpose

    Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (YU2) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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