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Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
Ward JD
Genetics. 2014 Dec 9. pii: genetics.114.172361.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61250pJW1219CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans
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61251pJW1259Cas9 plasmid with sgRNA deleted; for use in C. elegans
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61252pJW1285CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans
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61253pJW1310Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion
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61254pJW1311Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion
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