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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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253,513 results

Showing: 5241 - 5260 of 253513 results

  1. pAAV.TBG.PI.eGFP.WPRE.bGH

    Plasmid
    #105535
    Purpose
    AAV expression of EGFP from TBG promoter
    Depositor
    James M. Wilson
    Has service
    AAV8
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TBG
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105535-plasmid-map-sequence-id-202369

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  2. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid
    #100854
    Purpose
    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jRGECO1a
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100854-plasmid-map-sequence-id-466906

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  3. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid
    #100854
    Purpose
    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jRGECO1a
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100854-plasmid-map-sequence-id-466906

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  4. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Publication
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87854-plasmid-map-sequence-id-175127

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  5. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Publication
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87854-plasmid-map-sequence-id-175127

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  8. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71667-plasmid-map-sequence-id-243369

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  9. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71667-plasmid-map-sequence-id-243369

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  16. pX330-Cetn1/1

    Plasmid
    #50718
    Purpose
    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Publication
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50718-plasmid-map-sequence-id-75953

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  17. pX330-Cetn1/1

    Plasmid
    #50718
    Purpose
    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Publication
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50718-plasmid-map-sequence-id-75953

    Enlarge

Showing: 5241 - 5260 of 253513 results