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Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
Nucleic Acids Res. 2013 Jun 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46569pdCas9Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
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46570pWJ66Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
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46571pWJ68Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
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