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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
(Plasmid #60815)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60815 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHMGWA
  • Total vector size (bp) 10076
  • Modifications to backbone
    additional of 4 His to 6X His for higher Ni-NTA affinity
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9 M1C D10A C80S H840A C574S
  • Species
    S. pyogenes
  • Mutation
    M1C D10A C80S H840A C574S
  • Tags / Fusion Proteins
    • His10 (N terminal on insert)
    • MBP (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S was a gift from Jennifer Doudna (Addgene plasmid # 60815 ; http://n2t.net/addgene:60815 ; RRID:Addgene_60815)
  • For your References section:

    Programmable RNA recognition and cleavage by CRISPR/Cas9. O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, Doudna JA. Nature. 2014 Sep 28. doi: 10.1038/nature13769. 10.1038/nature13769 PubMed 25274302