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GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.
Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
Plant Physiol. 2013 Jul;162(3):1618-31.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68161pUPD2Universal domesticator plasmid for level 0 parts
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68162pPAt2S3 (GB0029)Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part
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68163pP35S (GB0030)Provides the CaMV 35S Promoter as a level 0 GoldenBraid part
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68164pPML1 (GB0045)Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part
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68165pNH (GB0064)Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part
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68166pPnos (GB0072)Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part
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68167pPMtb (GB0080)Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part
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68168pP2A11 (GB0082)Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part
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68169pPTctp (GB0083)Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part
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68170pPUbc (GB0145)Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part
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68171pHsp18.2 (GB0153)Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part
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68172pHsp70B (GB0155)Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part
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68173p2x35S (GB0222)Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part
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68174pPAtUbq10 (GB0223)Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part
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68175pPromE8 (GB0914)Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part
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68176pUPD_Mini35s(-60,UTR) (GB0050)Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part
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68177pPAct2noUTR (GB0192)Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part
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68178p5'UTRAct2 (GB0193)Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part
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68179pOpLacI:mini35ss (GB0534)Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part
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68180pOpLexA::mini35s (GB0733)Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part
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68181pLexAOp (GB0118)Provides the 4xLexA Operator as a level 0 GoldenBraid part
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68182p6xUAS (GB0179)Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part
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68183pLacIBD::SDRX (GB0673)Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part
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68184pLexA::SRDX (GB0732)Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part
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68185pGal4BD::BDR (GB0734)Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part
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68186pTHsp18.2 (GB0035)Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part
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68187pT35S (GB0036)Provides the CaMV 35S terminator as a level 0 GoldenBraid part
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68188pTnos (GB0037)Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part
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68189pT2A11 (GB0143)Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part
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68190pTE8 (GB0144)Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part
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68191pTAct2 (GB0210)Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part
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68192pTAtUbq3 (GB0219)Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part
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68193pBFP (GB0025)Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part
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68194pRosea (GB0026)Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part
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68195pP19 (GB0038)Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part
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68196pHcPro (GB0067)Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part
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68197pYFP (GB0053)Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part
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68198pRenilla (GB0056)Provides the CDS of the Renilla protein as a level 0 GoldenBraid part
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68199pMyb12 (GB0075)Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part
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68200pDelila (GB0079)Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part
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68201pLuciferase (GB0096)Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part
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68202pDsRed (GB0100)Provides the CDS of DsRed as a level 0 GoldenBraid part
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68203pGUS (GB0208)Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part
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68204pHygromycin (GB0211)Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part
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68206phumanCas9 (GB0575)Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part
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68207pD10AH840AhCas9 (GB1041)Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
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68208pU6-26 (GB1001)Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part
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68209pUPD_OsU3 (GB1184)Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part
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68210psgRNA (GB0645)Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site
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68211pTNos:BASTA:PNos (GB0023)Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.
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68212pEGB Tnos:NptII:Pnos (GB0226)Transcriptional unit for kanamycin resistance gene plant expression
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68213pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)Transcriptional unit for Hygromycin resistance gene plant expression
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68214pDGB3alpha2_35S:P19:Tnos (GB1203)Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter
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68215pEGB 35S:Renilla:Tnos (GB0109)Transcriptional unit for Renilla Protein plant expression driven by the 35S promoter
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68216pEGB 35S:Luciferase:Tnos (GB0110)Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter
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68217pEGB Pnos:Luciferase:Tnos (GB0112)Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter
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68218pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
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68219pEGB 35S:YFP:Tnos (GB0209)Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter
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68220pEGB 35S:DsRed:Tnos (GB0361)Transcriptional unit for DsRed plant expression driven by the 35S promoter
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68222pEGB2alpha2 35s:hCas9:tNos (GB0639)Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter
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68223pEGB 35S:dCas9:Tnos (GB1191)Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
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68224pDGB1_alpha1GoldenBraid level 1 Alpha plant expression vector.
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68225pDGB1_alpha2GoldenBraid level 1 Alpha plant expression vector.
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68226pDGB1_alpha1RGoldenBraid level 1 Alpha plant expression vector.
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68227pDGB1_alpha2RGoldenBraid level 1 Alpha plant expression vector.
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68228pDGB3_alpha1GoldenBraid level 1 Alpha plant expression vector.
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68229pDGB3_alpha2GoldenBraid level 1 Alpha plant expression vector.
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68230pDGB3_alpha1RGoldenBraid level 1 Alpha plant expression vector.
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68231pDGB3_alpha2RGoldenBraid level 1 Alpha plant expression vector.
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68232pDGB1alpha1_SF (GB0106)Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector
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68233pDGB1alpha2_SF (GB0107)Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector
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68234pDGB1_omega1GoldenBraid level 1 Omega plant expression vector
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68235pDGB1_omega2GoldenBraid level 1 Omega plant expression vector
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68236pDGB1_omega1RGoldenBraid level 1 Omega plant expression vector
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68237pDGB1_omega2RGoldenBraid level 1 Omega plant expression vector
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68238pDGB3_omega1GoldenBraid level 1 Omega plant expression vector
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68239pDGB3_omega2GoldenBraid level 1 Omega plant expression vector
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68240pDGB3_omega1RGoldenBraid level 1 Omega plant expression vector
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68241pDGB3_omega2RGoldenBraid level 1 Omega plant expression vector
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68242pDGB1omega1_SF (GB0158)Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector
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68243pDGB1omega2_SF (GB0466)Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector
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