GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.
Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
Plant Physiol. 2013 Jul;162(3):1618-31. PubMed Article
Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
Plant Physiol. 2013 Jul;162(3):1618-31. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68161 | pUPD2 | Universal domesticator plasmid for level 0 parts |
| 68162 | pPAt2S3 (GB0029) | Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part |
| 68163 | pP35S (GB0030) | Provides the CaMV 35S Promoter as a level 0 GoldenBraid part |
| 68164 | pPML1 (GB0045) | Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part |
| 68165 | pNH (GB0064) | Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part |
| 68166 | pPnos (GB0072) | Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part |
| 68167 | pPMtb (GB0080) | Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part |
| 68168 | pP2A11 (GB0082) | Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part |
| 68169 | pPTctp (GB0083) | Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part |
| 68170 | pPUbc (GB0145) | Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part |
| 68171 | pHsp18.2 (GB0153) | Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part |
| 68172 | pHsp70B (GB0155) | Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part |
| 68173 | p2x35S (GB0222) | Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part |
| 68174 | pPAtUbq10 (GB0223) | Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part |
| 68175 | pPromE8 (GB0914) | Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part |
| 68176 | pUPD_Mini35s(-60,UTR) (GB0050) | Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part |
| 68177 | pPAct2noUTR (GB0192) | Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part |
| 68178 | p5'UTRAct2 (GB0193) | Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part |
| 68179 | pOpLacI:mini35ss (GB0534) | Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part |
| 68180 | pOpLexA::mini35s (GB0733) | Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part |
| 68181 | pLexAOp (GB0118) | Provides the 4xLexA Operator as a level 0 GoldenBraid part |
| 68182 | p6xUAS (GB0179) | Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part |
| 68183 | pLacIBD::SDRX (GB0673) | Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part |
| 68184 | pLexA::SRDX (GB0732) | Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part |
| 68185 | pGal4BD::BDR (GB0734) | Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part |
| 68186 | pTHsp18.2 (GB0035) | Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part |
| 68187 | pT35S (GB0036) | Provides the CaMV 35S terminator as a level 0 GoldenBraid part |
| 68188 | pTnos (GB0037) | Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part |
| 68189 | pT2A11 (GB0143) | Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part |
| 68190 | pTE8 (GB0144) | Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part |
| 68191 | pTAct2 (GB0210) | Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part |
| 68192 | pTAtUbq3 (GB0219) | Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part |
| 68193 | pBFP (GB0025) | Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part |
| 68194 | pRosea (GB0026) | Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part |
| 68195 | pP19 (GB0038) | Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part |
| 68196 | pHcPro (GB0067) | Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part |
| 68197 | pYFP (GB0053) | Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part |
| 68198 | pRenilla (GB0056) | Provides the CDS of the Renilla protein as a level 0 GoldenBraid part |
| 68199 | pMyb12 (GB0075) | Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part |
| 68200 | pDelila (GB0079) | Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part |
| 68201 | pLuciferase (GB0096) | Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part |
| 68202 | pDsRed (GB0100) | Provides the CDS of DsRed as a level 0 GoldenBraid part |
| 68203 | pGUS (GB0208) | Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part |
| 68204 | pHygromycin (GB0211) | Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part |
| 68206 | phumanCas9 (GB0575) | Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part |
| 68207 | pD10AH840AhCas9 (GB1041) | Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part |
| 68208 | pU6-26 (GB1001) | Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part |
| 68209 | pUPD_OsU3 (GB1184) | Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part |
| 68210 | psgRNA (GB0645) | Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site |
| 68211 | pTNos:BASTA:PNos (GB0023) | Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector. |
| 68212 | pEGB Tnos:NptII:Pnos (GB0226) | Transcriptional unit for kanamycin resistance gene plant expression |
| 68213 | pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235) | Transcriptional unit for Hygromycin resistance gene plant expression |
| 68214 | pDGB3alpha2_35S:P19:Tnos (GB1203) | Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter |
| 68215 | pEGB 35S:Renilla:Tnos (GB0109) | Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter |
| 68216 | pEGB 35S:Luciferase:Tnos (GB0110) | Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter |
| 68217 | pEGB Pnos:Luciferase:Tnos (GB0112) | Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter |
| 68218 | pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116) | Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression |
| 68219 | pEGB 35S:YFP:Tnos (GB0209) | Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter |
| 68220 | pEGB 35S:DsRed:Tnos (GB0361) | Transcriptional unit for DsRed plant expression driven by the 35S promoter |
| 68222 | pEGB2alpha2 35s:hCas9:tNos (GB0639) | Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter |
| 68223 | pEGB 35S:dCas9:Tnos (GB1191) | Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter |
| 68224 | pDGB1_alpha1 | GoldenBraid level 1 Alpha plant expression vector. |
| 68225 | pDGB1_alpha2 | GoldenBraid level 1 Alpha plant expression vector. |
| 68226 | pDGB1_alpha1R | GoldenBraid level 1 Alpha plant expression vector. |
| 68227 | pDGB1_alpha2R | GoldenBraid level 1 Alpha plant expression vector. |
| 68228 | pDGB3_alpha1 | GoldenBraid level 1 Alpha plant expression vector. |
| 68229 | pDGB3_alpha2 | GoldenBraid level 1 Alpha plant expression vector. |
| 68230 | pDGB3_alpha1R | GoldenBraid level 1 Alpha plant expression vector. |
| 68231 | pDGB3_alpha2R | GoldenBraid level 1 Alpha plant expression vector. |
| 68232 | pDGB1alpha1_SF (GB0106) | Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector |
| 68233 | pDGB1alpha2_SF (GB0107) | Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector |
| 68234 | pDGB1_omega1 | GoldenBraid level 1 Omega plant expression vector |
| 68235 | pDGB1_omega2 | GoldenBraid level 1 Omega plant expression vector |
| 68236 | pDGB1_omega1R | GoldenBraid level 1 Omega plant expression vector |
| 68237 | pDGB1_omega2R | GoldenBraid level 1 Omega plant expression vector |
| 68238 | pDGB3_omega1 | GoldenBraid level 1 Omega plant expression vector |
| 68239 | pDGB3_omega2 | GoldenBraid level 1 Omega plant expression vector |
| 68240 | pDGB3_omega1R | GoldenBraid level 1 Omega plant expression vector |
| 68241 | pDGB3_omega2R | GoldenBraid level 1 Omega plant expression vector |
| 68242 | pDGB1omega1_SF (GB0158) | Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector |
| 68243 | pDGB1omega2_SF (GB0466) | Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector |