PurposeProvides the CaMV dual 35S Promoter as a level 0 GoldenBraid part
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68173||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerself-made; derived from pGEMT-Easy manufactured by Promega
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2x35S promoter
Insert Size (bp)1329
MutationBsaI and BsmBI sites removed
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2x35S (GB0222) was a gift from Diego Orzaez (Addgene plasmid # 68173 ; http://n2t.net/addgene:68173 ; RRID:Addgene_68173)
For your References section:GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. Plant Physiol. 2013 Jul;162(3):1618-31. 10.1104/pp.113.217661 PubMed 23669743