PurposeProvides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68211||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerself-made; derived from pGEMT-Easy manufactured by Promega
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namebar gene
Insert Size (bp)1146
MutationBsaI and BsmBI sites removed
- Promoter PNos
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTNos:BASTA:PNos (GB0023) was a gift from Diego Orzaez (Addgene plasmid # 68211 ; http://n2t.net/addgene:68211 ; RRID:Addgene_68211)
For your References section:GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. Plant Physiol. 2013 Jul;162(3):1618-31. 10.1104/pp.113.217661 PubMed 23669743