PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68206||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerself-made; derived from pGEMT-Easy manufactured by Promega
Vector typeCRISPR, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCas9 coding region (human codon optimised)
Insert Size (bp)4137
MutationBsaI and BsmBI sites removed; human codon optimised
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:phumanCas9 (GB0575) was a gift from Diego Orzaez (Addgene plasmid # 68206 ; http://n2t.net/addgene:68206 ; RRID:Addgene_68206)
For your References section:GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. Plant Physiol. 2013 Jul;162(3):1618-31. 10.1104/pp.113.217661 PubMed 23669743