pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)
PurposeTranscriptional unit for Hygromycin resistance gene plant expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68213||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerself-made; derived from pGreenII generated at the JIC
Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)1811
MutationBsaI and BsmBI sites removed
- Promoter Pnos
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer caacctctcgggcttctgga (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
insert can be released with BsmBI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235) was a gift from Diego Orzaez (Addgene plasmid # 68213 ; http://n2t.net/addgene:68213 ; RRID:Addgene_68213)
For your References section:GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. Plant Physiol. 2013 Jul;162(3):1618-31. 10.1104/pp.113.217661 PubMed 23669743