pEGB 35S:dCas9:Tnos (GB1191)
PurposeTranscriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68223||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerself-made; derived from pCambia1302 generated at the Cambia Institute
Vector typePlant Expression, CRISPR, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)5664
MutationBsaI and BsmBI sites removed; human codon optimised; mutated (D10A, H840A) and inactivated catalytic domains
- Promoter 35S
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer GGTGGCAGGATATATTGTGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
insert can be released with BsmBI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGB 35S:dCas9:Tnos (GB1191) was a gift from Diego Orzaez (Addgene plasmid # 68223 ; http://n2t.net/addgene:68223 ; RRID:Addgene_68223)
For your References section:GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. Plant Physiol. 2013 Jul;162(3):1618-31. 10.1104/pp.113.217661 PubMed 23669743