|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49771||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition and placed it under the 35S promoter using the Golden Gate system from S. Marillonnet.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST was a gift from Sophien Kamoun (Addgene plasmid # 49771 ; http://n2t.net/addgene:49771 ; RRID:Addgene_49771)
For your References section:Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V. Plant Methods. 2013 Oct 11;9(1):39. 10.1186/1746-4811-9-39 PubMed 24112467