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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST
(Plasmid #49771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49771 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 49771-D50 50 μg of DNA in Tris buffer $495
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DNA 49771-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pICH47742
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    35Sp::hCas9
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hCas9 from George Church

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition and placed it under the 35S promoter using the Golden Gate system from S. Marillonnet.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST was a gift from Sophien Kamoun (Addgene plasmid # 49771 ; http://n2t.net/addgene:49771 ; RRID:Addgene_49771)

  • For your References section:

    Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V. Plant Methods. 2013 Oct 11;9(1):39. 10.1186/1746-4811-9-39 PubMed 24112467
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