Purpose(Empty Backbone) Please see depositor comments section.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48015||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4824
Selectable markersCanthaxanthin operon
Growth in Bacteria
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
PLEASE NOTE- It has recently come to light that plasmid pAGM4723 lacks a complete overdrive sequence at the right border. This can cause incomplete TDNA insertion events and can give a higher incidence of vector backbone transfer events due to transfer initiating at the left border.
Level 2 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells.
Please note that bacterial colonies for this plasmid often appear red in color because the plasmid contains a red color selectable marker (CRed--an artificial bacterial operon responsible for canthaxanthin biosynthesis).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAGM4723 was a gift from Sylvestre Marillonnet (Addgene plasmid # 48015 ; http://n2t.net/addgene:48015 ; RRID:Addgene_48015)
For your References section:A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. 10.1371/journal.pone.0016765 PubMed 21364738