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A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.
Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, Zheng X, Hsieh TF, Qi Y
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69273pYPQ131AGolden Gate entry vector; 1st gRNA under AtU6 promoter
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69274pYPQ132AGolden Gate entry vector; 2nd gRNA under AtU6 promoter
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69275pYPQ133AGolden Gate entry vector; 3rd gRNA under AtU6 promoter
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69276pYPQ134AGolden Gate entry vector; 4th gRNA under AtU6 promoter
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69277pYPQ135AGolden Gate entry vector; 5th gRNA under AtU6 promoter
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69278pYPQ136AGolden Gate entry vector; 6th gRNA under AtU6 promoter
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69279pYPQ137AGolden Gate entry vector;7th gRNA under AtU6 promoter
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69280pYPQ138A Golden Gate entry vector; 8th gRNA under AtU6 promoter
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69281pYPQ131BGolden Gate entry vector; 1st gRNA under AtU3 promoter
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69282pYPQ132BGolden Gate entry vector; 2nd gRNA under AtU3 promoter
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69283pYPQ133B Golden Gate entry vector; 3rd gRNA under AtU3 promoter
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69284pYPQ131CGolden Gate entry vector; 1st gRNA under OsU6 promoter
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69285pYPQ132CGolden Gate entry vector; 1st gRNA under OsU6 promoter
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69286pYPQ133CGolden Gate entry vector; 1st gRNA under OsU6 promoter
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69287pYPQ131DGolden Gate entry vector; 1st gRNA under OsU3 promoter
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69288pYPQ132DGolden Gate entry vector; 1st gRNA under OsU3 promoter
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69289pYPQ133DGolden Gate entry vector; 1st gRNA under OsU3 promoter
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69290pYPQ141AGateway entry vector; single gRNA under AtU6 promoter
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69291pYPQ141BGateway entry vector; single gRNA under AtU3 promoter
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69292pYPQ141CGateway entry vector; single gRNA under OsU6 promoter
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69293pYPQ141DGateway entry vector; single gRNA under OsU3 promoter
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69294pYPQ142Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs
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69295pYPQ143Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs
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69296pYPQ144Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs
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69297pYPQ145 Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs
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69298pYPQ146Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs
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69299pYPQ147Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs
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69300pYPQ148Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs
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69301pYPQ150Gateway entry vector with pcoCas9 (Plant codon optimized)
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69302pYPQ151Gateway entry vector with pcoCas9D10A
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69303pYPQ152Gateway entry vector with pco-dCas9-VP64
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69304pYPQ153Gateway entry vector with pco-dCas9-3X(SRDX)
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69305pYPQ154Gateway entry vector with AteCas9 (Arabidopsis codon optimized)
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69306pYPQ155Gateway entry vector with AteCas9D10A
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69307pYPQ158Gateway entry vector with hSpCas9 (Human codon optimized)
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69308pYPQ159Gateway entry vector with hSpCas9D10A
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69309pYPQ167Gateway entry vector with Cas9p
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