PurposeGateway entry vector with pco-dCas9-3X(SRDX)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69304||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturer(modified from pNJB185)
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namepco-dCas9-3X(SRDX) (Plant codon-optimized)
MutationSRDX (X3) repressor fusion; NLS fusion
/ Fusion Protein
- 2XFLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site AatII (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYPQ153 was a gift from Yiping Qi (Addgene plasmid # 69304 ; http://n2t.net/addgene:69304 ; RRID:Addgene_69304)
For your References section:A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, Zheng X, Hsieh TF, Qi Y. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. 10.1104/pp.15.00636 PubMed 26297141