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pAdTrack-CMV FLAG Rev-Erb alpha
(Plasmid #22745)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22745 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAdTrack-CMV
  • Backbone manufacturer
    Available at Addgene (#16405)
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rev Erb alpha
  • Alt name
    Rev-Erbalpha
  • Species
    H. sapiens (human)
  • Entrez Gene
    NR1D1 (a.k.a. EAR1, THRA1, THRAL, ear-1, hRev)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdTrack-CMV FLAG Rev-Erb alpha was a gift from Bruce Spiegelman (Addgene plasmid # 22745 ; http://n2t.net/addgene:22745 ; RRID:Addgene_22745)
  • For your References section:

    PGC-1alpha negatively regulates hepatic FGF21 expression by modulating the heme/Rev-Erb(alpha) axis. Estall JL, Ruas JL, Choi CS, Laznik D, Badman M, Maratos-Flier E, Shulman GI, Spiegelman BM. Proc Natl Acad Sci U S A. 2009 Dec 29. 106(52):22510-5. 10.1073/pnas.0912533106 PubMed 20018698