|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27489||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneMSCV2.2 (MIG)
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
/ Fusion Proteins
- ECMV (C terminal on insert)
- IRES (C terminal on insert)
- NGFR (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
MSCV ires NGFR (referred to as "NGFR") is identical to MigR1 except “ires GFP” of Mig R1 is replaced by the ECMV ires cloned in frame to the truncated human nerve growth factor receptor (tNGFR), which encodes the extracellular and transmembrane domains of hNGFR.
The source of the LTRs in this plasmid (and MigR1) is Pear et al., Blood. 1998 Nov 15;92(10):3780-92, which cites the MSCV2.2 plasmid from Hawley et al., Gene Ther. 1994 Mar;1(2):136-8. The MSCV LTRs are present at the following locations (numbering according to the full plasmid sequence obtained by Addgene):
5' LTR - bp# 2474-2990
3' LTR - bp# 5389-5903
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NGFR was a gift from Warren Pear (Addgene plasmid # 27489 ; http://n2t.net/addgene:27489 ; RRID:Addgene_27489)
For your References section:Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, Pui JC, Koretzky GA, Pear WS. Immunity. 2001 Mar . 14(3):253-64. 10.1016/S1074-7613(01)00107-8 PubMed 11290335