MSCV-IRES-Thy1.1 DEST
(Plasmid #17442)

• Purpose: (Empty Backbone) Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction
• Depositing Lab: Anjana Rao
• Publication: Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Backbone

• Vector backbone: MSCV-IRES-Thy1.1
• Backbone manufacturer: Philippa Marrack Lab
• Backbone size (bp): 6063
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Thy1.1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: pBABE5'
• 3′ sequencing primer: IRES-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 29 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.