|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17442||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerPhilippa Marrack Lab
- Backbone size (bp) 6063
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site attB1 (not destroyed)
- 3′ cloning site attB2 (not destroyed)
- 5′ sequencing primer pBABE5'
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-IRES-Thy1.1 DEST was a gift from Anjana Rao (Addgene plasmid # 17442 ; http://n2t.net/addgene:17442 ; RRID:Addgene_17442)
For your References section:FOXP3 controls regulatory T cell function through cooperation with NFAT. Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A. Cell. 2006 Jul 28. 126(2):375-87. 10.1016/j.cell.2006.05.042 PubMed 16873067