pCE-BiFC-VN173
(Plasmid #22019)

• Depositing Lab: Chang-Deng Hu
• Publication: Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Backbone

• Vector backbone: pPD4983
• Backbone manufacturer: Fire lab 1995 kit
• Backbone size w/o insert (bp): 3993
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VN173
• Alt name: Venus(1-172)
• Species: Aequorea Victoria
• Insert Size (bp): 522
• Mutation: N/A
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Hiatt, S.M., Shyu, Y., Duren, H.M, and Hu, C.D. Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in living C. elegans. Methods, 45:185-191 (2008)

Multicloning sites betwee Myc and VN173 are avaialble