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Addgene

pCMV6 BDNF
(Plasmid #39857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6-XL3
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BDNF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    750
  • GenBank ID
    X55573
  • Entrez Gene
    Bdnf
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6 BDNF was a gift from Yves-Alain Barde (Addgene plasmid # 39857 ; http://n2t.net/addgene:39857 ; RRID:Addgene_39857)

  • For your References section:

    Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain. Hofer M, Pagliusi SR, Hohn A, Leibrock J, Barde YA. EMBO J. 1990 Aug;9(8):2459-64. PubMed 2369898
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