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A split-Cas9 architecture for inducible genome editing and transcription modulation.
Zetsche B, Volz SE, Zhang F
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62883PX851N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).
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62884PX852C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
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62885PX853N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).
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62886PX854C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
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62887PX855N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
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62888PX856C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
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62889pLSC-5Lenti vector for expression of inducible split Cas9
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