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C321.∆A.opt

Bacterial strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Publication

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
FUW-tetO-wtYAP

Plasmid

#84009

Purpose

expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Publication

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGWB401

Plasmid

#74795

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ hnRNPK myc HIS

Plasmid

#71662

Purpose

Mammalian expression of hnRNPK

Depositor

Gideon Dreyfuss

Insert

hnRNPK (HNRNPK Human)

Tags

HIS and myc

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1_iSH2-pMag(3x)-iRFP

Plasmid

#67304

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

iSH2-pMag(3x)-iRFP (PIK3R1 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Publication

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PX855

Plasmid

#62887

Purpose

N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Aspartic acide 10 to Alanine (D10A)

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Publication

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCitrine-pBAD

Plasmid

#54723

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Publication

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and mCitrine

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pLKO.1-puro U6 sgRNA BfuAI large stuffer

Plasmid

#52628

Purpose

lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

Depositor

Scot Wolfe

Insert

Kanamycin cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pCMV6 BDNF

Plasmid

#39857

Depositor

Yves-Alain Barde

Publication

Hofer et al EMBO J. 1990 Aug;9(8):2459-64.

Insert

BDNF (Bdnf Mouse)

Expression

Mammalian

Promoter

CMV

Available since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICH47751

Plasmid

#48002

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

15 Articles

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pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Publication

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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MSCV-CMV-DsRed-IRES-EGFP-DEST

Plasmid

#41941

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP(N3)-PP1beta

Plasmid

#44223

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1B (PPP1CB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-hCaM

Plasmid

#47602

Depositor

Emanuel Strehler

Insert

CaM (CALM3 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC4 (XRCC4 Human)

Tags

GFP-2xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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