166,818 results

1. Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF)

   Pooled Library

   #1000000113

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of mouse genes

   Depositor

   John Doench, David Root

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pSR33.4r

   Plasmid

   #63198

   Purpose

   Expresses Cph8, Ho1, and PcyA constitutively

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   Cph8
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHN924

   Plasmid

   #178885

   Purpose

   Expression of GST-His8-HRV3C

   Depositor

   Kazuhiro Abe

   Insert

   human rhinovirus 3C protease
   

   Use

   Tags

   GST, His8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEG BacMam

   Plasmid

   #160451

   Purpose

   vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-tTA

   Plasmid

   #104102

   Purpose

   Expresses tTA2 under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. sfGFP-N1

   Plasmid

   #54737

   Purpose

   Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

   Depositor

   Michael Davidson, Geoffrey Waldo

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHel3

   Plasmid

   #102961

   Purpose

   E.coli/H.Pylori shuttle vector

   Depositor

   Rainer Haas

   Article

   Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO.Sox9.Puro

   Plasmid

   #117269

   Purpose

   Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A and puromycin resistant cassette (C terminal …

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENN.AAV.hSyn.Cre.WPRE.hGH

   Plasmid

   #105553

   Purpose

   AAV expression of Cre from hSyn promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 acGFP

    Plasmid

    #128047

    Purpose

    Mammalian expression vector with N terminal AcGFP

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.2-ccdB-Nanoluc

    Plasmid

    #87075

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mouse sgRNA library Brie in lentiCRISPRv2

    Pooled Library

    #73632

    Purpose

    Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCAGGS_3E5E_eGFP

    Plasmid

    #103054

    Purpose

    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    

    Use

    Tags

    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)

    Viral Prep

    #130995-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (#130995). In addition to the viral particles, you will also receive purified pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. hSyn-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Tags

    mScarlet

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

15. TFORF3550

    Plasmid

    #145026

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Mariner2_AG

    Plasmid

    #217135

    Purpose

    Mammalian expression vector for expression of Mariner2_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    Mariner2_AG transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAct:dCas9-VPR

    Plasmid

    #78898

    Purpose

    Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Flag Runx1 FL (P#1790)

    Plasmid

    #14585

    Depositor

    Matija Peterlin

    Article

    Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. AttB_mCherry

    Plasmid

    #171598

    Purpose

    Strong mCherry fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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