168,579 results

1. pmiRFP720-N1

   Plasmid

   #136560

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GZnP3 in pcDNA3.1+

   Plasmid

   #161738

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

   Insert

   GZnP3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet-1∆HisTag_hNMT_Nef

   Plasmid

   #66078

   Purpose

   Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

   Depositor

   Dieter Willbold

   Article

   Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

   Inserts

   NMT (NMT1 Human)
   Nef
   

   Expression

   Bacterial

   Mutation

   Deletion of first 80 amino acids

   Promoter

   T7-lacO

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1a-BbChT (AAV9)

   Viral Prep

   #45186-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Promoter

   EF1a

   Tags

   mCherry, mTFP, or none

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

5. mTurquoise2-N1

   Plasmid

   #54843

   Purpose

   Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

   Depositor

   Michael Davidson, Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Type

   Empty backbone

   Tags

   mTurquoise2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP-N2-COL1A1

   Plasmid

   #66602

   Purpose

   Expresses procollagen 1 alpha 1 as a GFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Expression

   Mammalian

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHygCG2

   Plasmid

   #200073

   Purpose

   hygR selection mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2MLS-HyperNova/pcDNA3

   Plasmid

   #221457

   Purpose

   Expressing HyperNova in mitochondria

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
   

   Expression

   Mammalian

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Promoter

    T7

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Mm.cargo(Cre)

    Plasmid

    #174862

    Purpose

    Expresses mouse Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by MmPeg10 UTRs
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBPnlsLexA::p65Uw

    Plasmid

    #26230

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::p65 (RELA Human)
    

    Expression

    Insect

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. phage UbiC scAB-GFP

    Plasmid

    #104998

    Purpose

    Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    single chain Antibody against GCN4
    

    Use

    Lentiviral

    Tags

    GB1, HA tag, and sfGFP

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_OT1.0

    Plasmid

    #185386

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKC-4.06

    Plasmid

    #112084

    Purpose

    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Human, Photinus pyralis)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX317-EGFP

    Plasmid

    #115439

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKC-4.04

    Plasmid

    #112085

    Purpose

    Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin (HBB Mouse, Human, Photinus pyralis)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    K3753R

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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