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(Plasmid #82415)


Item Catalog # Description Quantity Price (USD)
Plasmid 82415 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    lentiCRISPR v2 (Addgene #52961)
  • Backbone manufacturer
    Feng Zhang
  • Total vector size (bp) 13459
  • Modifications to backbone
    Cloned in Cre to replace Puro resistance
  • Vector type
    Lentiviral, Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    Cre Recombinase
  • Alt name
  • Species
    P1 Phage
  • Insert Size (bp)
  • Mutation
    Mutated BsmBI cut site
  • Promoter EFS (P2A)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sgRNA of choice is cloned into vector using Golden Gate assembly, with BsmBI restriction enzyme (as per resource information on Addgene plasmid #52961 by Feng Zhang).

sgRNA sequencing primer is as follows: 5'-TACGTGACGTAGAAAGTA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPRv2Cre was a gift from David Feldser (Addgene plasmid # 82415 ; ; RRID:Addgene_82415)
  • For your References section:

    Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma. Walter DM, Venancio OS, Buza EL, Tobias JW, Deshpande C, Gudiel AA, Kim-Kiselak C, Cicchini M, Yates TJ, Feldser DM. Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159. 10.1158/0008-5472.CAN-16-2159 PubMed 28202515