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LentiCRISPRv2GFP
(Plasmid #82416)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82416 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang
  • Total vector size (bp) 13129
  • Modifications to backbone
    Cloned in GFP to replace Puro resistance
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    EGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720
  • Promoter EFS (P2A)

Cloning Information

Resource Information

Depositor Comments

sgRNA of choice is cloned into vector using Golden Gate assembly, with BsmBI restriction enzyme (as per resource information on Addgene plasmid #52961 by Feng Zhang). Note: This plasmid does not contain a 2KB stuffer sequence between the BsmBI sites.

sgRNA sequencing primer is as follows: 5'-TACGTGACGTAGAAAGTA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPRv2GFP was a gift from David Feldser (Addgene plasmid # 82416)
  • For your References section:

    Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma. Walter DM, Venancio OS, Buza EL, Tobias JW, Deshpande C, Gudiel AA, Kim-Kiselak C, Cicchini M, Yates TJ, Feldser DM. Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159. 10.1158/0008-5472.CAN-16-2159 PubMed 28202515